Binding Equilibria

Calculation of the Association Constant (Ka)

Calculation of the Dissociation Constant (Kd)

Relation between binding affinity and free energy

In the simplest cast, suppose we have an interaction between a protein and a ligand,

[P] + [L] ⇌ [P:L]

[P] denotes the concentration of the protein

[L] denotes the concentration of the ligand

[P:L] denotes the concentration of the protein-ligand complex

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